a novel low molecular weight extracellular protease from a moderately halophilic bacterium salinivibrio sp. strain ms-7: production and biochemical properties
نویسندگان
چکیده
kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, salinivibrio sp. strain ms-7, and maximum growth and protease activity was achieved after 48 hours at 30°c and 180 rpm. to determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and maximum production of the enzyme was obtained in a basal medium (ph 8.0) containing maltose as a carbon source (494 u/ml). the protease was isolated from a stationary phase culture, purified 3.6-fold with 56% yield by a simple procedure and characterized biochemically. the enzyme revealed a monomeric structure with a relative molecular mass of 21 kda by running on sds-page. maximum caseinolytic activity of the enzyme was observed at 50°c, ph 8.0 and 0–0.5 m nacl with a high tolerance to salt concentrations of up to 3 m. the effect of various metal ions and inhibitors on caseinolytic activity of the purified protease revealed that it probably belongs to the subclass of serine metalloproteases. these findings suggest that the protease secreted by salinivibrio sp. strain ms-7 can be introduced as a candidate for biotechnological applications based on its haloalkaline properties.
منابع مشابه
A novel low molecular weight extracellular protease from a moderately halophilic bacterium Salinivibrio sp. strain MS-7: production and biochemical properties
Kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, Salinivibrio sp. strain MS-7, and maximum growth and protease activity was achieved after 48 hours at 30°C and 180 rpm. To determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and maximum production of...
متن کاملHalomonas kribbensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea.
A moderately halophilic, Gram-negative bacterium, designated strain BH843(T), was isolated from a solar saltern in Korea and subjected to a taxonomic analysis. Strain BH843(T) grew at salinities of 1-14 % (w/v) NaCl and at temperatures of 10-40 degrees C. The cells were motile cocci or short rods with single flagella and contained C(16 : 0), C(19 : 0) cyclo omega8c and C(17 : 0) cyclo as the ma...
متن کاملMarinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment.
Two Gram-negative, cold-adapted, moderately halophilic, aerobic bacteria, designated strains M3B(T) and M3T, were isolated from marine sediment collected from the South Shetland Islands, Antarctica. The organisms were rod-shaped, catalase- and oxidase-positive, and motile by means of polar flagella. These two psychrotolerant strains required Na(+) and grew at NaCl concentrations of 1-15 % and t...
متن کاملExtracellular proteases of Halobacillus blutaparonensis strain M9, a new moderately halophilic bacterium
Halophilic microorganisms are source of potential hydrolytic enzymes to be used in industrial and/or biotechnological processes. In the present study, we have investigated the ability of the moderately halophilic bacterium Halobacillus blutaparonensis (strain M9), a novel species described by our group, to release proteolytic enzymes. This bacterial strain abundantly proliferated in Luria-Berta...
متن کاملEnhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate Salicola marasensis
Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was ...
متن کاملGenome Sequence of Martelella sp. Strain AD-3, a Moderately Halophilic Polycyclic Aromatic Hydrocarbon-Degrading Bacterium
Martelella sp. strain AD-3, enriched from a petroleum-contaminated site with high salinity, can efficiently degrade polycyclic aromatic hydrocarbons. Here, we report the 4.75-Mb genome sequence of strain AD-3 with its genetic feature of helping to remediate environmental organic pollutants.
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
molecular biology research communicationsناشر: shiraz university press
ISSN 2322-181X
دوره 1
شماره 2 2012
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023